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KMID : 0613820080180060902
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Journal of Life Science
2008 Volume.18 No. 6 p.902 ~ p.906
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Analysis of Microsatellite DNA Polymorphisms for Pedigree Verification in Kyungju Dog(Dongkyung-i)
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Lee Eun-Woo
Choi Seog-Gyu
Cho Gil-Jae
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Abstract
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In this study, we analyzed the microsatellite DNA polymorphisms for pedigree verification in Kyungju dog (Dongkyung-i) which is one of the Korean breed dogs. A total of 51 Dongkyung-i samples were genotyped using 8 microsatellite markers. The number of alleles observed at single locus ranged from 4 to 12, with average number of alleles per locus of 8.5. The expected heterozygosity and polymorphic information contents (PIC) values of the 8 micro-satellite loci were 0.6162～0.8746 (mean 0.7587) and 0.5461～0.8512 (mean 0.7167), respectively. Of the 8 markers, PEZ3, PEZ6, PEZ12 and FHC2054 loci had relatively high PIC values (＞0.7) in Dongkyung-i. Pedigree verification of Dongkyung-i was analyzed based on alleles observed. The results of the parent-age testing were noted significant differences compared with breeders. These results show basic in-formation of conservation and research in Dongkyung-i, and further studies of genetic pedigree in Dongkyung-i will be needed.
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KEYWORD
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Dongkyung-i, microsatelliteDNApolymorphisms, pedigreeverification
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